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目的:分析糖尿病足患者血清基质金属蛋白酶2(MMP-2)、MMP-9总水平和活性水平变化及临床意义。方法:在本院住院的糖尿病足病患者80例作为糖尿病足组(DF组),同期住院无足病糖尿病患者80例为非糖尿病足组(NDF组),年龄、性别匹配的健康人80例作为对照组(CON组)。ELISA法测定各组血清MMP-2和MMP-9总水平,明胶酶谱法检测各组血清MMP-2、MMP-9活性水平。多因素回归分析影响MMP的危险因素。结果:CON、NDF和DF各组血清MMP-2、MMP-9总水平依次升高(P均<0.05)。DF组和NDF组MMP-2活性高于CON组(P均<0.05),DF组和NDF组水平差异无统计学意义(P>0.05);DF组MMP-9活性水平高于CON组和NDF组(P均<0.05)。多元回归分析显示,DF、糖化血红蛋白(HbA1c)、病程和年龄是MMP-9活性升高的危险因素。结论:糖尿病患者血清MMP-2和MMP-9总水平和活性升高,是DF发生和发展的重要因素。
Objective: To analyze the changes of serum MMP-2, MMP-9 levels and their activity in patients with diabetic foot and its clinical significance. Methods: Eighty diabetic diabetic foot patients hospitalized in our hospital were enrolled in the diabetic foot group (DF group), 80 non-diabetic foot patients (NDF group) and 80 matched healthy and age-matched healthy patients As a control group (CON group). Serum levels of MMP-2 and MMP-9 were measured by ELISA. Serum levels of MMP-2 and MMP-9 were detected by gelatin zymography. Multivariate regression analysis of risk factors affecting MMP. Results: The levels of serum MMP-2 and MMP-9 in CON, NDF and DF groups all increased successively (all P <0.05). The activities of MMP-2 in DF group and NDF group were higher than those in CON group (P <0.05), but there was no significant difference between DF group and NDF group (P> 0.05) Group (all P <0.05). Multiple regression analysis showed that DF, HbA1c, duration of disease and age were the risk factors of MMP-9 activity. Conclusion: The overall level and activity of serum MMP-2 and MMP-9 in diabetic patients is an important factor in the occurrence and development of DF.