本文将percoll分离的兔肾近端小管细胞,应用微细胞培养插盘,在Ham F 12和Dulbecco’s modified Eagle混合培养液中进行双室培养。应用充氧的培养液培养3周后,获得一层完整的近端小管细胞。透射电镜观察表明,细胞具有近端小管细胞的特点,如微绒毛的出现、较丰富的线粒体、吞饮小泡、溶酶体以及不规则的质膜内褶。~(14)C标记的葡萄糖吸收试验,以及“~(14)CO_2释放的测定,说明培养出的一层近端小管细胞重吸收功能完好,代谢旺盛。本文并对该方法进行了较详细的介绍和分析。 In this paper, percoll isolated rabbit renal proximal tubule cells were cultured in a double chamber in Ham F 12 and Dulbecco’s modified Eagle mixed medium using a microtiter culture plate. After 3 weeks of culture in oxygenated medium, a complete proximal tubular cell was obtained. Transmission electron microscopy showed that the cells had the characteristics of proximal tubule cells such as the presence of microvilli, abundant mitochondria, vesicles, lysosomes and irregular plaques. ~ (14) C-labeled glucose uptake assay and the determination of "~ (14) CO 2 release indicated that the cultured proximal tubular cells had intact and metabolically strong reabsorption.In this paper, a more detailed Introduction and analysis.