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为提高硝化菌的亚硝酸盐氧化能力,利用统计试验设计(Plackett-Burman和Box-Behnken设计)优化得到一最佳培养基:NaHCO32.0g·L-1;NaNO22.36g·L-1;Na2CO30.37g·L-1;NaCl0.34g·L-1;KH2PO40.05g·L-1;MgSO4·7H2O0.05g·L-1;FeSO.47H2O0.03g·L-1。在此条件下,硝化菌的最大亚硝酸盐氧化速率达到905.0mgNO2-N·(gMLSS.d)-1(mixed liquor suspended solids,MLSS,混合液悬浮固体)。将50L降解速率为850mgNO2-N·(gMLSS,d)-1的硝化菌(浓度为1.99gVSS·L-1)(volatile solid,VSS,挥发性固体)投加至0.6hm2的养殖水体中,7d内试验水体中的亚硝酸盐浓度即降至安全浓度以下。
In order to improve the nitrite oxidation ability of nitrifying bacteria, an optimal medium was obtained by using statistical experimental design (Plackett-Burman and Box-Behnken design): NaHCO32.0g · L-1; NaNO22.36g · L-1; Na2CO30 .37 g · L -1; NaCl 0.34 g · L -1; KH 2 PO 40.05 g · L -1; MgSO 4 · 7H 2 O 0.05 g · L -1; FeSO 4 7H 2 O 0.03 g · L -1. Under these conditions, the maximum nitrite oxidation rate of nitrifying bacteria reached 905.0mgNO2-N · (gMLSS.d) -1, mixed liquor suspended solids (MLSS). 50L of nitrifying bacteria (volatile solid, VSS, volatile solid) with a degradation rate of 850mgNO2-N · (gMLSS, d) -1 was added to 0.6hm2 of aquaculture water for 7d The nitrite concentration in the test water drops below the safe concentration.