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目的 探讨加温逆转肿瘤细胞株ＫＢＶ２００ 多药耐药性及与诱导细胞凋亡的关系。方法用四唑蓝快速比色法（ＭＴＴ法） 检测细胞存活率；用形态学（ 光镜、电镜观察） ；生化学（ＤＮＡ 末端转移标记法，ＴＵＮＥＬ法） 及流式细胞术分析细胞凋亡状况。结果 加温４２ ℃，１ ｈ 可明显降低ＫＢＶ２００ 细胞存活率，提高长春新碱细胞毒性作用；单加ＶＣＲ 细胞存活率为（９０ ．７０ ±８．７８） ％ ，加ＶＣＲ 加温为（５７ ．４０±２１ ．５５）％ ，Ｐ＜ ０ ．００２。加温与细胞分化剂ＣＤＡ－ Ⅱ合用，有明显协同逆转作用。ＣＤＡ－ Ⅱ与ＶＣＲ合用细胞存活率为（５９ ．２４ ±３０．８０）％ ，加温与ＣＤＡ－ Ⅱ及ＶＣＲ 合用为（１５ ．３２ ±２ ．６８）％ ，Ｐ＜ ０．０５。４２ ℃或４３ ℃处理１，２４ ｈ 后即可观察到明显细胞凋亡。４３ ℃１ ｈ 处理诱导作用更强。结论 加温可明显逆转肿瘤细胞多药耐药及诱导细胞凋亡。加温对肿瘤细胞凋亡的诱导，在加温逆转肿瘤细胞耐药性中可能起着重要作用。
Objective To investigate the relationship between the multidrug resistance of KBV200 cell line and the induction of cell apoptosis. Methods The viability of cells was detected by MTT assay; apoptosis was analyzed by morphology (light microscopy, electron microscopy); biochemistry (DNA terminal metastasis labeling, TUNEL method) and flow cytometry. situation. The results of heating 42 °C, 1 h can significantly reduce the survival rate of KBV200 cells and enhance the cytotoxic effect of vincristine; single plus VCR cell survival rate was (90.70 ± 8.78)%, plus VCR heating (57. 40±21.55)%, P< 0 . 002. Heating combined with CDA-II, a cell differentiation agent, has a significant synergistic reversal effect. The survival rate of CDA-II combined with VCR cells was (59.24 ± 30.80)%, and the combination of heating with CDA-II and VCR was (15.32 ± 2.68)%, P < 0.05. 42 °C After treatment for 1 or 24 h at 43 °C, significant apoptosis was observed. 43 °C 1 h treatment induced stronger. Conclusion Heating can significantly reverse the multidrug resistance of tumor cells and induce apoptosis. The induction of tumor cell apoptosis by warming may play an important role in reversing the drug resistance of tumor cells by warming.