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目的利用大鼠脊髓连续切片,结合三维重建工具软件对正常大鼠皮质脊髓束进行三维重建。方法制作正常成年SD大鼠脊髓连续横断切片,进行Luxol快蓝(LFB)染色并摄片,获得大鼠脊髓皮质脊髓束连续切片图像。在Photoshop软件环境下完成切片图像的配准、分割、灰度化。利用3D-DOCTOR软件进行阈值分割并对大鼠脊髓、脊髓灰质、双侧皮质脊髓束结构进行三维重建,在普通台式机(PC)上对重建结构进行任意角度的观察、切割和测量。结果重建出的大鼠脊髓、脊髓灰质、双侧皮质脊髓束具有立体感,大鼠皮质脊髓束于大鼠颈1节段(C1)~骶4节段(S4)走行于大鼠脊髓后索腹侧,紧贴灰质中间带背侧,呈逐渐变细的类圆柱状形态。在PC机中进行切割后可以对切割后剩余结构进行表面积和体积的数据测量。结论利用大鼠脊髓的连续LFB染色切片,结合计算机三维重建技术,能够进行大鼠皮质脊髓束的三维重建,并提供测量数据。
OBJECTIVE: To establish a three-dimensional reconstruction of cortical spinal cord bundles in rats using serial sections of rat spinal cord and three-dimensional reconstruction software. Methods Serial spinal cord sections of normal adult SD rats were made and stained with Luxol Fast Blue (LFB) and photographed to obtain serial images of spinal cords of spinal cord in rats. In the Photoshop software environment to complete the registration of slice images, segmentation, grayscale. Using 3D-DOCTOR software for threshold segmentation and three-dimensional reconstruction of spinal cord, polio and bilateral corticospinal tract structures in rats, the reconstructed structures were observed, cut and measured at any angle on a common PC. Results The reconstructed rat spinal cord, the gray matter of the spinal cord and the bilateral cortical spinal cord bundle had stereopsis. The corticospinal tracts of rats clumped to the posterior spinal cord of rats in the segments C1-C4 of the cervical spine (S4) The ventral side, close to the gray dorsal medulla, was tapered cylinder-like morphology. After cutting in the PC, the remaining structure after cutting can be surface area and volume data measurements. Conclusions Using continuous LFB staining of spinal cord in rats and 3D reconstruction of computer, three-dimensional reconstruction of rat corticospinal tract can be performed and the measurement data provided.