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以中肋骨条藻为对象，研究了铁以及铁与氮的交互作用对藻类生长、色素化程度及氮同化能力的影响。结果表明，在３×１０￣８～５×１０－６ｍｏｌ／ｄｍ３浓度范围内，随着Ｆｅ的增加，该藻对氮的吸收量增加，同化能力增大，细胞色素化程度降低，胞内氨基酸（ＡＡ）和蛋白质（Ｐｒ）含量升高，细胞生长加快，细胞生长率在Ｆｅ浓度为５×１０－６ｍｏｌ／ｄｍ３时达最大。叶绿素ａ和褐色素以及胞内ＡＡ和Ｐｒ与铁浓度的对数呈正相关关系。实验还发现，当Ｎ／Ｐ为２０：１时（比较４０：１和１０：１），铁与氮交互作用对细胞生长、色素化程度以及氮同化能力影响尤其显著：当Ｆｅ浓度一定时，不同形态的氮源对藻胞内ＡＡ转化为Ｐｒ速率的影响是低氧化态氮源高于高氧化态氮源
With Skeletonema costatum as an object, the effects of iron, iron and nitrogen interactions on algal growth, pigmentation and nitrogen assimilation were studied. The results showed that in the range of 3 × 10 ~ 8 ~ 5 × 10-6mol / dm3, the uptake of nitrogen increased with the increase of Fe, the assimilation capacity increased, the degree of cytochromes decreased, the intracellular amino acids (AA) and protein (Pr) increased, cell growth accelerated, cell growth rate reached the maximum when Fe concentration was 5 × 10-6mol / dm3. Chlorophyll a and brown pigment and intracellular AA and Pr were positively correlated with the logarithm of iron concentration. It was also found that the interaction between iron and nitrogen had a significant effect on the cell growth, the degree of pigmentation and the nitrogen assimilation ability when the N / P was 20: 1 (compared with 40: 1 and 10: 1). When the Fe concentration was constant, The effect of different forms of nitrogen sources on the rate of conversion of AA to Pr in algal cells is that the nitrogen source at low oxidation state is higher than the nitrogen source at high oxidation state