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本研究通过3年4环境的田间试验,在BIL(backcross inbred lines)群体中选取断裂比强度最大的材料NMGA-062(32.5cN/tex)和断裂比强度最小的材料NMGA-144(26.67cN/tex),利用Affymetrix棉花基因芯片,比较分析NMGA-062和NMGA-144纤维发育10d时的基因表达谱。在24029条转录本中,两个基因型中差异表达的转录本6504条,占筛选转录本总数的27.07%;其中差异表达倍数在2倍或2倍以上的转录本有3461条,占筛选转录本总数的14.41%,对其进行功能分类。对8个差异表达明显的基因(Ghi.10655.1.S1_s_at,ACO1,ARF1,SAHH,TUA6,TUA7,β-tub1,β-tub10)进行实时荧光定量PCR的检测,发现表达模式均与芯片结果一致,说明芯片数据具有生物学意义上的可重复性,结果是可信的。本研究为以后克隆及功能验证纤维发育相关基因提供了重要参考。
In this study, NMGA-062 (32.5cN / tex) and NMGA-144 (26.67cN / tex) were selected as materials with the highest breaking strength in the BIL (backcross inbred lines) tex), gene expression profiles of NMGA-062 and NMGA-144 fibers at 10 days of development were compared using Affymetrix cotton gene chips. Among 24029 transcripts, 6504 transcripts differentially expressed in the two genotypes accounted for 27.07% of the total number of transcripts screened, among which 3461 transcripts were doubled or more than 2 times, The total number of 14.41%, its functional classification. Real-time fluorescence quantitative PCR was performed on eight differentially expressed genes (Ghi.10655.1.S1_s_at, ACO1, ARF1, SAHH, TUA6, TUA7, β-tub1 and β-tub10) This shows that the chip data is biologically repeatable and the result is credible. This study provides an important reference for future cloning and functional verification of fiber development related genes.