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目的:研究乌司他丁对心肺复苏后早期脑水肿和脑超微结构的变化的影响。方法:建立大鼠心肺复苏模型,120只大鼠随机分成对照组、复苏组和药物组(每组再按时间分即刻、1/2 h、3 h、6 h、9 h五亚组),检测心肺复苏后各时相脑组织水含量及伊文氏兰(EB)含量的变化,以电镜观察各时相脑组织超微结构。结果:复苏组心肺复苏后1/2 h、3 h、6 h、9 h脑组织水含量持续增加,6 h后EB含量升高,电镜观察各时间点见细胞水肿,6 h后V-R间隙明显扩大;药物组仅于复苏后1/2、3 h有脑组织水含量的增加,6 h后水含量回降,各时间点EB含量无明显升高,复苏后6 h开始超微结构改变明显减少。结论:乌司他丁可通过抑制脑水肿,对大鼠心肺复苏后早期脑损伤起到一定的保护作用。
OBJECTIVE: To study the effect of ulinastatin on the changes of early brain edema and ultrastructure of brain after cardiopulmonary resuscitation. Methods: A rat model of cardiopulmonary resuscitation (CPR) was established. One hundred and twenty rats were randomly divided into control group, resuscitation group and drug group (each subgroup was divided into five subgroups at 1/2 h, 3 h, 6 h and 9 h) The changes of water content in brain tissue and Evans blue (EB) content after cardiopulmonary resuscitation (CPR) were detected. The ultrastructure of brain tissue was observed by electron microscopy. Results: After resuscitation, the water content of brain tissue kept increasing at 1/2 h, 3 h, 6 h, 9 h after cardiopulmonary resuscitation, and the content of EB was increased 6 h later. The cell edema was observed by electron microscopy at 6 h. The water content in brain tissue increased only 1/2 and 3 h after resuscitation, and the water content decreased after 6 h, the content of EB did not increase significantly at all time points, and the microstructure changed obviously at 6 h after resuscitation cut back. Conclusion: Ulinastatin can inhibit cerebral edema and protect the early brain injury after cardiopulmonary resuscitation in rats.