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目的研究极高频电磁复合波对晚期恶性肿瘤患者外周血辅助淋巴细胞亚群Th1/Th2免疫应答平衡的影响。方法对32例恶性肿瘤患者化疗后进行极高频电磁复合波幅照,并采用ELISA法检测幅照治疗前、后外周血中的IFN-γ和IL-4水平变化;另对30例恶性肿瘤化疗后患者,同比进行细胞因子检测;再分别对上述患者外周血进行IFN-γ和IL-4水平变化的自身对照研究,以评价极高频电磁复合波对恶性肿瘤患者化疗后免疫功能的影响。结果①恶性肿瘤化疗后第8天,患者IFN-γ水平(24.66±12.85)pg·mL-1低于化疗后第3天水平(27.88±17.07)pg·mL-1,但未见显著性差异(P>0.05);而IL-4水平(54.80±28.56)pg·mL-1则明显地高于化疗后第3天水平(44.97±27.53)pg·mL-1,P<0.05。②极高频电磁复合波幅照的化疗患者,化疗后第8天,IFN-γ水平(34.79±27.23)pg·mL-1远高于化疗后第3天水平(20.39±12.67)pg·mL-1,P<0.05;IL-4水平变化研究结果显示,化疗后第8天,患者的IL-4水平(43.49±34.04)pg·mL-1高于化疗后第3天水平(35.77±22.23)pg·mL-1,但其间差异无显著性(P>0.05)。③恶性肿瘤患者化疗后第3天至第8天,细胞因子IFN-γ/IL-4水平比值降低,其间有显著性差异(P<0.05);经极高频电磁复合波幅照后,其比值明显升高[从(0.57±0.44)pg·mL-1升至(0.80±0.67)pg·mL-1],P<0.05。结论恶性肿瘤患者化疗后第3天至第8天,细胞因子IFN-γ水平降低,而IL-4水平则明显升高,反映恶性肿瘤化疗后患者Th细胞的存在异常(Th2)漂移;但极高频电磁复合波幅照治疗,可干预或阻抑恶性肿瘤患者化疗后Th细胞的异常漂移。
Objective To study the effect of high frequency electromagnetic complex on the balance of Th1 / Th2 immune responses in peripheral blood lymphocytes in patients with advanced malignant tumors. Methods Thirty-two patients with malignant tumor were treated with radiofrequency (HF) electromagnetic wave following chemotherapy. The levels of IFN-γ and IL-4 in peripheral blood were detected by ELISA before and after radiotherapy. Thirty patients with malignant tumor Patients were compared cytokines compared to the same time; respectively, the above patients were peripheral blood IFN- and IL-4 levels of self-control study to evaluate the impact of high frequency electromagnetic wave on malignant tumor patients after chemotherapy immune function. Results ① On the 8th day after chemotherapy, the level of IFN-γ in patients with malignant tumor (24.66 ± 12.85) pg · mL-1 was lower than that of the third day after chemotherapy (27.88 ± 17.07) pg · mL-1, but no significant difference was found (P> 0.05). The level of IL-4 (54.80 ± 28.56) pg · mL-1 was significantly higher than that of the third day after chemotherapy (44.97 ± 27.53) pg · mL-1, P <0.05. At the 8th day after chemotherapy, the level of IFN-γ (34.79 ± 27.23) pg · mL-1 was much higher than that of the third day after chemotherapy (20.39 ± 12.67) pg · mL- 1, P <0.05. The results of IL-4 level change showed that the level of IL-4 (43.49 ± 34.04) pg · mL-1 on the 8th day after chemotherapy was higher than that on the 3rd day after chemotherapy (35.77 ± 22.23) pg · mL-1, but the difference was not significant (P> 0.05). ③The ratio of IFN-γ / IL-4 in the malignant tumor patients decreased from the 3rd day to the 8th day after chemotherapy, and there was a significant difference (P <0.05). The ratio of the IFN- Increased significantly from (0.57 ± 0.44) pg · mL-1 to (0.80 ± 0.67) pg · mL-1], P <0.05. Conclusions The levels of IFN-γ and IL-4 in patients with malignant tumor decreased from day 3 to day 8 after chemotherapy, reflecting the abnormal Th (Th2) shift in patients with malignant tumor after chemotherapy. However, High-frequency electromagnetic composite wave amplitude therapy can interfere with or suppress the abnormal migration of Th cells in patients with malignant tumors after chemotherapy.