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根据近年来流行病学资料分析表明,在原发性肝癌的致病因素中,以黄曲霉毒素B_1的致癌作用最为重要。有关黄曲霉毒素B_1的毒效测试方法,多采用Ames方法,其诱发突变的浓度为0.06微克/毫升。本文报道用姐妹染色单体互换(简称SCE)法测定黄曲霉毒素B_1对正常人淋巴细胞诱变效应的结果,以提供黄曲霉毒素B_1与肝癌发生的可能关系。方法:取自3例正常成人(男2例,女1例)的血样。参照Kornberg方法制成SCE片。培养液内含有RPMI 1640和小牛血清等成分。每毫升培养液内含30微克分子(或9微克/毫升)6-溴脱氧尿核苷(瑞士产品)。结果:当黄曲霉毒素B_1在培养液内的浓度分别为0.001微克/毫升和0.01微克/毫升时,平均每染色体的SCE值(即染色单体交换数与观察数之比)分别为0.08(61/725)和0.08(108/1276)。浓度为0.02微克/毫升时,各为0.18(160/906)、0.15(222/1520)和0.13(121/957),平均SCE值为0.15。浓度为0.04微克/毫升时各为0.20(130/661)、0.18(190/1062)和0.17(190/1148),平均为0.18。浓度为0.06微克/毫升时各为0.19(208/1096)、0.22(310/1429)和0.23(150/658),平均为0.21。讨论:由于癌变剂和突变剂是密切相关的。即在相当大的范围内两者为同一化学物质,因此测试突变的实验手
According to the analysis of epidemiological data in recent years, the carcinogenic effect of aflatoxin B 1 is the most important factor in the pathogenesis of primary liver cancer. The method of measuring the toxicity of aflatoxin B1 is mostly Ames method, and the concentration of its induced mutation is 0.06 μg/ml. This paper reports the results of the sister chromatid exchange (SCE) method for measuring the mutagenic effects of aflatoxin B1 on lymphocytes in normal humans to provide a possible relationship between aflatoxin B1 and liver cancer. Methods: Blood samples were taken from 3 normal adults (male 2 and female 1). The SCE sheet is made according to the Kornberg method. The culture contains components such as RPMI 1640 and calf serum. 30 μg (or 9 μg/ml) of 6-bromodeoxyuridine (Swiss product) per ml of culture medium. Results: When the concentration of aflatoxin B 1 in the culture fluid was 0.001 μg/ml and 0.01 μg/ml, the average SCE value per chromosome (ie, the ratio of chromatid exchange to observation) was 0.08 (61). /725) and 0.08 (108/1276). At a concentration of 0.02 μg/ml each was 0.18 (160/906), 0.15 (222/1520), and 0.13 (121/957), with an average SCE value of 0.15. At a concentration of 0.04 μg/ml each was 0.20 (130/661), 0.18 (190/1062), and 0.17 (190/1148), with an average of 0.18. At a concentration of 0.06 μg/ml each was 0.19 (208/1096), 0.22 (310/1429) and 0.23 (150/658), with an average of 0.21. Discussion: Because cancer agents and mutations are closely related. That is, within a fairly large range, both are the same chemical substance, so test mutant testers