昌红苹果离体叶片不定芽的诱导及植株再生

来源 :云南农业大学学报 | 被引量 : 0次 | 上传用户:vs1ji
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以昌红苹果的组培苗为试材,研究建立叶片的离体再生体系。用30—40d继代的新梢顶部1.3节新展开的叶片作为外植体,分别接种在分化的培养基上,黑暗处理15d,昌红苹果叶片不定芽的最高再生率为100%。在6号培养基上,昌红苹果叶片的再生能力最高;诱导叶片不定芽适宜的培养基为MS+TDZ1.0mg/L+NAA0.5mg/L+蔗糖30g/L+琼脂5.5g/L。
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