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目的:建立氨酚比林注射液中对乙酰氨基酚、氨基比林含量的高效液相色谱方法。方法:选用AgilentSBC18(150 mm ×4．6mm,5μm),乙腈-0．1％磷酸溶液-三乙胺(12:87:1)为流动相,检测波长为240 nm,流速为1．0 ml·min-1。结果:对乙酰氨基酚的线性范围为0．35-1．74μg(r=0．999 9),平均回收率为101．4％,RSD=0．5％;氨基比林的线性范围为0．48～2．42 μg,r=0．999 9,平均回收率为100．3％,RSD=0．4％。结论:本方法快速、准确,样品处理简便易行。
OBJECTIVE: To establish a HPLC method for the determination of acetaminophen and aminopyrine in acetaminophen injection. Methods: The mobile phase consisted of AgilentSBC18 (150 mm × 4.6 mm, 5 μm), acetonitrile-0.1% phosphoric acid solution-triethylamine (12: 87: 1) with a detection wavelength of 240 nm and a flow rate of 1.0 ml · Min-1. Results: The linear range of acetaminophen was 0.35-1.74μg (r = 0.999 9), the average recovery was 101.4% and the RSD was 0.5%. The linear range of aminopyrine was 0 .48 ~ 2.42 μg, r = 0.999 9, the average recovery was 100.3%, RSD = 0.4%. Conclusion: The method is rapid and accurate, and the sample processing is simple and easy.