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目的直肠癌位于直肠内,是人类常见的消化道恶性肿瘤之一。癌干细胞理论的提出,使人们对肿瘤发生发展有了一个全新的认识。根据癌干细胞的理论,直肠癌组织内存在的少量癌干细胞,最终导致了肿瘤治疗后的复发和转移。通过癌干细胞的表面标记物,可帮助确定癌干细胞,并且研究标记物在直肠癌与正常直肠组织表达的不同,以及它的表达与不同临床病理特点之间的关系。在本实验中,对直肠癌患者组织标本中的癌干细胞标记物CD133进行研究。方法采用免疫组织化学法检测CD133蛋白表达与直肠癌临床病理参数之间的关系,及它在癌组织和正常直肠组织中表达的不同特点。结果与正常直肠组织相比,直肠癌组织中CD133蛋白表达水平明显升高。它的表达还与肿瘤Duke’s分期和TNM分期有明显的相关性。临床分期越高,肿瘤组织表达的CD133染色率越高。而且相对于无淋巴结转移组,在有淋巴结转移的样本组,CD133的表达率明显升高。结论人直肠癌中,癌干细胞标记物CD133可对患者的临床病理特点做出评估,说明它在肿瘤的形成过程中起到一定的作用。
Rectal cancer is located in the rectum, is one of the common human gastrointestinal cancer. The proposal of cancer stem cell theory, make people have a new understanding of tumor development. According to the theory of cancer stem cells, a small amount of cancer stem cells present in rectal cancer tissues eventually lead to recurrence and metastasis after tumor treatment. The use of surface markers of cancer stem cells helps identify cancer stem cells and examines the differences in the expression of markers between rectal cancer and normal rectal tissue and the relationship between its expression and different clinicopathological features. In this experiment, cancer stem cell marker CD133 in rectal cancer tissue samples was studied. Methods The relationship between the expression of CD133 protein and the clinicopathological parameters of rectal cancer was analyzed by immunohistochemistry. The expression of CD133 protein in cancer tissues and normal rectal tissues was analyzed by immunohistochemistry. Results Compared with normal rectal tissue, CD133 protein expression was significantly increased in rectal cancer tissues. Its expression was also significantly correlated with Duke’s stage and TNM stage. The higher the clinical stage, the higher the staining rate of CD133 expressed in tumor tissue. Moreover, the expression of CD133 was significantly higher in the group of lymph node metastasis than in the group without lymph node metastasis. Conclusion In human rectal cancer, CD133, a marker of cancer stem cells, can evaluate the clinicopathological features of the patients, indicating that it plays a role in tumor formation.