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目的:探讨脑络欣通方对大脑中动脉闭塞模型大鼠缺血再灌注(MCAO/R)损伤后大鼠血管新生及局部脑血流量(r CBF)的影响。方法:Wistar大鼠90只随机分为假手术组、模型组和脑络欣通组,采用线栓法制作MCAO/R模型,每组分为3,7,14 d共3个时间点的亚组,酶联免疫吸附试验(ELISA)检测脑组织匀浆中内皮素(ET)和血小板生成素(TPO)水平,免疫组化法检测脑组织中血管内皮生长因子(VEGF)的表达,运用激光多普勒仪动态观察r CBF。结果:与假手术组比较,模型组TPO和ET水平明显升高,VEGF蛋白表达增加,r CBF降低;与模型组比较,脑络欣通组TPO和ET水平显著降低,VEGF蛋白表达增加,r CBF明显恢复;同组内比较,脑络欣通组第7天和第14天较第3天TPO水平显著下降,第7天和第14天ET水平较第3天明显下降,第14天较第3天VEGF蛋白表达增加,第14天r CBF较第3天显著增加。结论:脑络欣通冻干粉通过调节模型大鼠凝血纤溶调节因子和VEGF,促进血管新生而改善血流状态,提高局部脑血流量来发挥对缺血性脑血管病的治疗作用。
Objective: To investigate the effects of Naoluoxintong Recipe on angiogenesis and regional cerebral blood flow (r CBF) in rats with middle cerebral artery occlusion (MCAO / R) injury after ischemia / reperfusion (MCAO / R) injury. Methods: Ninety Wistar rats were randomly divided into sham operation group, model group and Naoluo Xintong group. The MCAO / R model was established by thread occlusion method. Each group was divided into three groups The levels of endothelin (ET) and thrombopoietin (TPO) in brain homogenate were detected by enzyme linked immunosorbent assay (ELISA). The expression of vascular endothelial growth factor (VEGF) in brain tissue was detected by immunohistochemistry. Doppler dynamic observation r CBF. Results: Compared with the sham operation group, the levels of TPO and ET in model group were significantly increased, the expression of VEGF protein was increased and r CBF was decreased. Compared with model group, the levels of TPO and ET were significantly decreased and the expression of VEGF protein increased CBF was significantly recovered; within the same group comparison, the brain group on the 7th and 14th day than the third day TPO levels significantly decreased the level of ET on the 7th and 14th day compared with the 3rd day significantly decreased on the 14th day On day 3, the expression of VEGF protein increased, and on the 14th day r CBF increased significantly compared with the third day. Conclusion: Naoluoxint freeze-dried powder can improve ischemic cerebrovascular disease by regulating coagulation and fibrinolytic regulatory factors and vascular endothelial growth factor (VEGF), promote angiogenesis and improve blood flow state, and improve local cerebral blood flow.