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在一种由N－3，5二硝基苯甲酸苯基甘氨酸衍生而成的Pirkle1-A型手性固定相上分离百树菊酯的8个立体异构体，通过选用不同的醇作为流动相的强溶剂，研究二元体系中流动相组分对保留值和选择性的影响。结果表明：对同一种组分的流动相，增加流动相的强度会缩短溶质的保留时间，且对非对映体的选择性影响比较大，而对对映体的选择性影响却不大，作为强溶剂的醇的结构不同会对异构体的分高度及峰形产生影响，二级醇或三级醇作强溶剂时的分高度好于一级醇，小分子醇作强溶剂时的峰形好于较大分子醇。
Eight stereoisomers of cystathionine were isolated on a Pirkle 1-A type chiral stationary phase derived from phenylglycine N-3,5-dinitrobenzoate by using different alcohols as the mobile Phase of the strong solvent to study the binary system of mobile phase components on retention and selectivity. The results showed that increasing the mobile phase intensity of the mobile phase with the same component shortens the retention time of the solute and has a greater influence on the selectivity of the diastereomer but not the selectivity of the enantiomer. The structure of alcohol as a strong solvent will affect the height and peak shape of the isomers. When the secondary alcohol or tertiary alcohol is a strong solvent, the sub-height is better than that of the primary alcohol. When the small-molecule alcohol is a strong solvent Peak shape is better than larger molecule alcohol.