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目的探讨咪达唑仑对大鼠肝脏缺血再灌注时细胞凋亡的影响及其保护作用。方法成年健康SD大鼠54只,随机分为3组:假手术组(Sham组)、缺血再灌注组(IR组)、咪达唑仑组(M组),每组18只。制作70%肝脏缺血再灌注模型,实验结束后即刻取肝左叶做标本。用免疫组织化学法检测Bcl-2与Bax蛋白表达量,TUNEL法检测肝细胞凋亡指数(AI)。结果与Sham组比较,各组肝组织Bcl-2、Bax蛋白含量、凋亡指数差异有统计学意义(P<0.05);与IR组比较,M组Bcl-2蛋白表达增加、Bax蛋白表达和AI减少(P<0.05)。IR、M组各组内各时相以再灌注6h最高。结论咪达唑仑可以通过调节Bcl-2、Bax蛋白表达,使肝细胞凋亡减轻,从而对大鼠缺血再灌注肝脏有一定的保护作用。
Objective To investigate the effect of midazolam on cell apoptosis during hepatic ischemia-reperfusion in rats and its protective effect. Methods Fifty-four adult SD rats were randomly divided into three groups: sham operation group (Sham group), ischemia reperfusion group (IR group) and midazolam group (M group), with 18 rats in each group. 70% hepatic ischemia-reperfusion model was made. After the experiment, the left lobe of the liver was taken as the specimen. The expression of Bcl-2 and Bax protein were detected by immunohistochemistry and the apoptosis index (AI) by TUNEL method. Results Compared with Sham group, the contents of Bcl-2, Bax and the apoptosis index in liver tissue in each group were significantly different (P <0.05). Compared with IR group, the expression of Bcl-2 protein, AI decreased (P <0.05). IR, M groups within each group to reperfusion 6h highest. Conclusion Midazolam can reduce the apoptosis of hepatocytes by regulating the expression of Bcl-2 and Bax proteins, which may have a protective effect on the liver of ischemia-reperfusion rats.