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目的 :查明陕西延安地区是否为肾综合征出血热 ( HFRS)自然疫源地及新疫区。方法 :选择有疫情报告地区 ,进行人间疫情调查、健康人群特异性抗体检测 ;鼠间疫情调查在调查点野外和居民区 ,用夹夜法捕鼠 ,调查鼠种及鼠密度 ,采集鼠肺及血清标本。鼠肺冰冻切片用免疫荧光法检测汉坦病毒抗原 ;鼠血用间接免疫荧光法和酶联免疫法检测 Ig G抗体。结果 :对延安市防疫站 2 0 0 1年统计的 7例 HFRS患者进行流行病学调查 ,均属在当地感染 ;检测延安市洛川县交口河镇 2 4 5例人血标本汉坦病毒特异性 Ig G抗体 ,阳性 5 0例 ,人群隐性感染率为 2 0 .41 %。检测 2 2份鼠肺及鼠血标本 ,HV抗原均为阴性 ;HV抗体 IFA法阳性 1例 ,ELISA法阳性 5例。结论 :延安地区有 HFRS散发病例 ,洛川交口河镇健康人群、褐家鼠、小家鼠中有汉坦病毒感染 ,证实了延安市洛川县交口河镇为 HFRS自然疫源地及新疫区。
Objective: To determine whether Yan’an, Shaanxi Province is a natural epidemic area and a new epidemic area of hemorrhagic fever with renal syndrome (HFRS). Methods: The epidemic situation was selected to carry out the investigation of human epidemic situation and the detection of antibody specific to healthy people. The epidemiological survey among rats was conducted in the field and residential area of the investigation spot. Serum samples. Rat lung frozen sections were detected by immunofluorescence Hantavirus antigen; mouse blood by indirect immunofluorescence and enzyme-linked immunosorbent assay Ig G antibody. Results: The epidemiological investigation of 7 HFRS patients in Yan’an City epidemic prevention station in 2001 were all endemic. All of them were detected in 245 human blood samples in Jiaokouhe, Luochuan County, Yanan City. Sex Ig G antibody, positive 50 cases, the crowd latent infection rate was 20.41%. Twenty-two rat lung and rat blood samples were tested for negative HV antigen. HV antibody IFA method was positive in 1 case and ELISA method was positive in 5 cases. CONCLUSIONS: There are cases of HFRS excretion in Yan’an area, Hantavirus infection in healthy population of Raohe Town of Luokou, Rattus norvegicus and Mus musculus, which confirmed that the epidemic area of HFRS natural foci and new epidemic Area.