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为了解广州市儿童呼吸道支原体感染情况，用一条共同的上游引物，二条特异性的下游引物建立的ＰＣＲ方法能同时扩增ＭＰ的６９１ｂｐ和ＭＧ的４３８ｂｐ粘附因子基因片段，但不会扩增其他支原体和细菌的ＤＮＡ。以ＰＣＲ和分离培养同时检测了１６２份呼吸道感染患儿的咽拭标本，结果ＰＣＲ检测到１２份阳性ＭＰＤＮＡ，阳性率为７４％；从１份阳性标本分离培养并克隆出１株ＭＰ。二方法均未检测到ＭＧ。提示在广州市呼吸道感染患儿中存在ＭＰ的感染，所检测的患儿无ＭＧ感染。
To understand the prevalence of respiratory tract mycoplasma infection in children in Guangzhou, a common upstream primer and two specific downstream primers were used to amplify both the 691bp MP and the 438bp adhesion factor gene fragment of MG without amplifying the other Mycoplasma and bacterial DNA. A total of 162 swallowing swab specimens from children with respiratory tract infection were detected by PCR and isolation and culture. Results 12 positive MPDNA were detected by PCR, the positive rate was 74%. One MP was isolated and cultured from one positive specimen. Both methods did not detect MG. Prompt MP infection in children with respiratory tract infection in Guangzhou, the detected children without MG infection.