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微波辐射是一种已知能够引起DNA合成及结构发生改变的诱变因素。本文的研究目的旨在阐明有关特异染色体畸变微核的发生,并通过分析畸变染色体的结构及暴露特定的微波辐射强度的人淋巴细胞微核试验以检测微波辐射的遗传毒性作用。血液采自不吸烟、近期无电离及非电离辐射暴露史的健康人体。辐射源为一反射调速管(频率为7.7 GHz,发生器最大输出功率为2W)。人血淋巴细胞于22℃恒温条件下暴露于频率为7.7 GHz、功率密度分别为50,1000,3000 mW/m~2的微波辐射场10,30,60 min后,将其置于含植物凝集素的培养基中培养45 h,再加入秋水仙碱培养3 h。每一试样分析
Microwave radiation is a known mutagenic agent that causes changes in DNA synthesis and structure. The purpose of this study is to elucidate the occurrence of micronuclei related to idiosyncratic chromosome aberrations and to examine the genotoxic effects of microwave radiation by analyzing the structure of aberrant chromosomes and human lymphocyte micronucleus assays that expose specific microwave intensities. Blood was collected from non-smoking, no recent exposure to ionizing and non-ionizing radiation in healthy humans. The source of radiation is a reflected velocity-modulation tube (frequency 7.7 GHz, generator maximum output power 2 W). Human blood lymphocytes were exposed to a microwave radiation field with a frequency of 7.7 GHz and power densities of 50, 1000 and 3000 mW / m ~ 2 respectively for 10, 30 and 60 min at a constant temperature of 22 ° C, Cultured in medium for 45 h and then added colchicine for 3 h. Analysis of each sample