论文部分内容阅读
目的探讨慢性丙型肝炎肝组织病变与肝细胞凋亡的相互关系．方法采用免疫组化方法对比检测了３０例慢性丙型肝炎患者肝组织中的Ｆａｓ抗原（ＦａｓＡｇ）和Ｆａｓ配体（ＦａｓＬ）的表达．结果ＦａｓＡｇ的表达主要在肝细胞浆，尤以邻近“碎屑样坏死”的淋巴细胞浸润区的肝细胞为甚，而ＦａｓＬ主要表达于炎症明显区的淋巴细胞．慢性丙型肝炎肝组织中ＦａｓＡｇ和ＦａｓＬ表达的检出率分别为６６７％（２０／３０）和６３３％（１９／３０），ＦａｓＡｇ和ＦａｓＬ表达阳性组的肝组织病变比阴性组严重；而且，随着组织炎症活动程度的加重其ＦａｓＡｇ和ＦａｓＬ表达也明显增强（χ２分别为３１５５和２３４６，Ｐ＜００１）；此外，ＦａｓＡｇ和ＦａｓＬ的表达与患者的性别无关（χ２分别为０１２和００３，Ｐ＞００５），与患者的年龄和血清ＡＬＴ水平无关（ｔ值分别为１４２和１４４，Ｐ＞００５），但与血清总胆红素（ＴＢｉｌ）、清蛋白（Ａ）、Ａ／Ｇ和凝血酶原活动度（ＰＴＡ）有关（ｔ值分别为２２４和２３０，２１５和２０８，２１８和２０９，２５４和２４８，Ｐ＜００５）．结论ＣＴＬＦａｓＦａｓＬ介导的肝细胞凋亡在慢性丙型肝炎肝组织的炎症?
Objective To investigate the relationship between liver lesions and hepatocellular apoptosis in chronic hepatitis C patients. Methods The expression of Fas antigen (FasAg) and Fas ligand (Fas ligand) in liver tissues of 30 patients with chronic hepatitis C were detected by immunohistochemistry. Results The expression of FasAg was mainly in the liver cytoplasm, especially in the hepatocytes adjacent to the “debris-like necrosis” lymphocyte infiltration area, while FasL was mainly expressed in the lymphocytes in the area of inflammation. The positive rates of FasAg and FasL in liver tissues of chronic hepatitis C were 667% (20/30) and 633% (19/30), respectively. The positive rates of FasAg and FasL were higher in liver tissues than those in negative ones And the expression of FasAg and FasL was also significantly increased with the degree of inflammatory activity (χ2 = 3155 and 2346, respectively, P <001). In addition, the expression of FasAg and FasL was positively correlated with the gender (Χ2 = 012 and 003, respectively, P> 005), but not with patient’s age and serum ALT level (t = 1.42 and 1.44, P> 0.05) And serum total bilirubin (TBil), albumin (A), A / G and prothrombin activity (PTA) (t values were 2 24 and 2 30,2 15 and 2 08, 218 and 209, 254 and 248, P <005). Conclusion CTL Fas-FasL-mediated hepatocyte apoptosis in chronic hepatitis C liver inflammation?