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本研究利用TRAP分子标记对茉莉花的遗传多样性进行分析,从36对TRAP候选引物中筛选出25对多态性丰富的引物,以28份茉莉花基因组DNA为模板进行PCR分析,共获得148条带,其中136条具有明显的多态性,多态率为91.9%;每对引物扩增出3~8个多态性带,平均5.44个条带。28份茉莉花种质材料间遗传相似系数为0.37~0.92,平均值为0.65;UPGMA聚类分析表明在遗传相似系数0.64时,可将28份材料划分为五个聚类组。研究结果表明,TRAP标记在茉莉花材料中具有丰富的多态性,可以进一步应用该技术进行茉莉花遗传多样性及亲缘关系的研究。
In this study, we analyzed the genetic diversity of jasmine by using TRAP markers and screened 25 pairs of polymorphic primers from 36 pairs of TRAP candidate primers. PCR was carried out using 28 genomic DNAs of jasmine as template to obtain 148 bands , Of which 136 had obvious polymorphism with a polymorphism rate of 91.9%. Each pair of primers amplified 3 ~ 8 polymorphic bands with an average of 5.44 bands. The genetic similarity coefficient of 28 jasmine germplasm materials was 0.37 ~ 0.92 with an average of 0.65. UPGMA clustering analysis indicated that 28 genetic materials could be divided into five cluster groups at genetic similarity coefficient of 0.64. The results showed that the TRAP marker was rich in polymorphic material in jasmine, and the genetic diversity and genetic relationship of jasmine could be further studied using this technique.