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目的:探讨与研究珍宝丸对新生乳鼠脑缺氧保护作用的机制。方法:取新生Wistar乳鼠120只,雌雄不限,随机分为对照组、模型组和珍宝丸治疗组各40只。将新生Wistar大鼠置于8%O2+92%N2(V/V)的缺氧箱内建立新生鼠缺氧模型,在造模前每天1次连续7天灌胃给予生理盐水和珍宝丸,缺氧期间也同样剂量给药,分别于缺氧1 h后的12、24、48、72h时间点处死动物取脑组织待测。采用酶联免疫法检测不同时间点各组大鼠脑组织中细胞色素C和Caspase-3的蛋白含量。结果:与对照组比较,模型组细胞色素C、Caspase-3的蛋白含量均在缺氧后12 h时间点显著增高且24 h达高峰,差异有统计学意义(P<0.01);48、72 h时间点细胞色素C、Caspase-3的蛋白含量低于12 h时间点,但仍明显高于对照组,差异有统计学意义(P<0.01)。珍宝丸治疗组细胞色素C、Caspase-3的蛋白含量也均在缺氧后12 h时间点增高且24 h达高峰,与对照组差异有统计学意义(P<0.01);48、72 h时间点细胞色素C、Caspase-3的蛋白含量低于12 h时间点,与对照组差异无统计学意义(P>0.05)。模型组在各个时间点上细胞色素C、Caspase-3的蛋白含量均明显高于珍宝丸治疗组,差异有统计学意义(P<0.01)。结论:珍宝丸对乳鼠脑缺氧有很好的保护作用,其机制可能与下调脑组织中细胞色素C、Caspase-3的蛋白含量有关。
Objective: To explore and study the mechanism of Zhenbao Pill’s protective effects on neonatal neonatal rats with hypoxia. Methods: 120 newborn Wistar suckling mice were randomly divided into control group, model group and Zhenbao Pill group. The newborn Wistar rats were placed in 8% O2 + 92% N2 (V / V) hypoxia chamber to establish a neonatal rat model of hypoxia, once a day for 7 consecutive days prior to modeling by intragastric administration of saline and Zhenbao pills, Hypoxia during the same dose, respectively, 1 h after hypoxia at 12,24,48,72 h time point animals were sacrificed to be tested brain tissue. Enzyme-linked immunosorbent assay was used to detect the protein content of cytochrome C and Caspase-3 in rat brain tissue at different time points. Results: Compared with the control group, the protein levels of cytochrome C and Caspase-3 in model group increased significantly at 12 h after hypoxia and peaked at 24 h, with significant difference (P <0.01); 48 and 72 h at the time point, the protein content of cytochrome C and Caspase-3 was lower than that at 12 h, but still significantly higher than that of the control group (P <0.01). The protein contents of cytochrome C and Caspase-3 of Zhenbao Pill group also increased at 12 h after hypoxia and peaked at 24 h, which was significantly different from that of control group (P <0.01); 48 and 72 h The protein content of cytochrome C and Caspase-3 was lower than that at 12 h, but there was no significant difference between the two groups (P> 0.05). The protein levels of cytochrome C and Caspase-3 in model group at each time point were significantly higher than that of Zhenbao pill group (P <0.01). Conclusion: Zhenbao Pill has a good protective effect on cerebral hypoxia in neonatal rats, and its mechanism may be related to down-regulating the protein content of cytochrome C and Caspase-3 in brain tissue.