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目的观察红车轴草异黄酮对去卵巢骨质疏松大鼠体内雌激素和骨代谢生化指标的影响,初步探讨其作用机制。方法取60只6月龄雌性Wistar大鼠随机分为假手术组和5个去卵巢组:模型组、雌激素组(2.5 mg.kg-1)和红车轴草异黄酮高(90 mg.kg-1)、中(60 mg.kg-1)、低(30 mg.kg-1)剂量组,给药12周后处死大鼠,取血液、尿液进行相关生化指标的检测。结果红车轴草异黄酮对去卵巢导致的体重增加有对抗作用;显著降低骨质疏松大鼠血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)和骨钙素(BGP)水平,升高体内雌激素水平;显著降低尿钙、尿磷含量。结论红车轴草异黄酮对骨质疏松大鼠有防治作用,其机制可能与提高体内雌激素水平,抑制骨吸收,促进骨形成有关。
Objective To observe the effect of isoflavone on the biochemical indexes of estrogen and bone metabolism in ovariectomized rats and to explore its mechanism. Methods Sixty six-month-old female Wistar rats were randomly divided into sham operation group and ovariectomized group. The model group, estrogen group (2.5 mg.kg-1), and red clover isoflavone high (90 mg.kg (60 mg · kg-1) and low dose (30 mg · kg-1). After 12 weeks of administration, rats were sacrificed and blood and urine samples were collected for biochemical detection. Results The results showed that isoflavone could antagonize the weight gain of ovariectomized rats and significantly decrease the level of serum alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) and osteocalcin (BGP) in osteoporotic rats, Increase estrogen levels in the body; significantly reduce urinary calcium, urinary phosphorus. Conclusion Isoclavia isoflavones have preventive and therapeutic effects on osteoporosis in rats. The mechanism may be related to the increase of estrogen level, inhibition of bone resorption and promotion of bone formation.