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本文介绍一种以四甲基联苯胺(TMB)为基质的快速、简便和敏感的ELISA。因受光学测定系统的限制,ELISA 的吸光度值通常是测定低物的最大吸收峰值,从而本法的检测范围较放射免疫测定法狭窄。以TMB 作为供氢体用于ELISA 比色测定,其敏感性、精确性和无致癌作用均优于邻苯二胺(OPD)和2,2′-联氮基-双〔3-乙基-苯噻唑啉磺酸
This article describes a rapid, simple and sensitive ELISA using tetramethylbenzidine (TMB) as a substrate. Due to the limitation of the optical measurement system, the absorbance value of ELISA is usually the maximum absorption peak value of the low substance. Therefore, the detection range of this method is narrower than that of radioimmunoassay. TMB as a hydrogen donor for ELISA colorimetric assay, its sensitivity, accuracy and non-carcinogenic effects are better than o-phenylenediamine (OPD) and 2,2’-azino-bis [3-ethyl- Benzthiazoline sulfonic acid