目的获得大肠杆菌全长ａｓｄ基因。方法采用计算机引物设计软件，长模板ＰＣＲ扩增法及限制性内切酶酶切分析。结果设计一对Ｅ．ｃｏｌｉａｓｄ基因ＰＣＲ引物；经长模板ＰＣＲ扩增获得１５１０ｂｐＰＣＲ扩增片段；经限制性内切酶酶切分析，证明该片段与电脑查询的Ｅ．ｃｏｌｉａｓｄ基因酶切谱相同。结论以本文设计的引物用长模板ＰＣＲ扩增法得到的片段初步确认为Ｅ．ｃｏｌｉａｓｄ基因。 Objective To obtain the full-length asd gene of Escherichia coli. Methods Using computer primer design software, long template PCR amplification and restriction enzyme digestion analysis. Results of a pair of design. coliasd gene PCR primers; amplified by long template PCR amplified 1510bpPCR fragment; restriction enzyme digestion analysis showed that the fragment with the computer query E. The coliasd gene is the same. Conclusion The primers designed by this paper with long template PCR amplification of the fragment was initially identified as E. coli coliasd gene.