论文部分内容阅读
建立了糙米中灭瘟素残留量的亲水作用液相色谱-质谱联用测定方法。样品用80%甲醇/水提取,经MCX固相萃取小柱净化,以Waters HPLC BEH HILIC色谱柱(100mm×2.1mm,1.7μm)分离,采用UPLC-MS/MS多反应监测(MRM)正离子模式测定,外标法定量。灭瘟素最低检测浓度为0.02mg/kg,在0.02、0.05和0.20mg/kg 3个添加水平下平均回收率为78%~95%,相对标准偏差为2.9%~5.2%。
A method for the determination of residues of blasticidin in brown rice by liquid chromatography-mass spectrometry (LC-MS / MS) was established. The samples were extracted with 80% methanol / water, purified by a MCX cartridge, and separated on a Waters HPLC BEH HILIC column (100 mm × 2.1 mm, 1.7 μm) using UPLC-MS / MS multiple reaction monitoring Mode determination, external standard method. The minimum detectable concentration of plaquetin was 0.02 mg / kg. The average recoveries of three concentrations at the concentrations of 0.02, 0.05 and 0.20 mg / kg were 78% -95% and the relative standard deviations were 2.9% -5.2%.