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目的研究酒精性肝损伤大鼠模型建立的理想方式。方法取健康雄性SD大鼠30只,随机分为对照组、模型组各15只,模型组给予高脂饲料并灌服白酒,持续四周。实验结束时从大鼠眼眶静脉丛采血,测定血清中谷氨酸氨基转移酶(ALT)、天门冬氨酸转移酶(AST)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL-C)、肝脏中超氧化物岐化酶(SOD)、丙二醛(MDA)、肝内糖原含量、肝细胞凋亡情况、肝脏系数。结果模型组动物的肝脏指数、血清中的TC、TG和HDL-C、肝糖原、肝脏中MDA的含量、SOD的含量与对照组比较,显著性升高(P<0.05);模型组动物血清中的ALT和AST与对照组比较,显著性升高(P<0.01);模型组动物肝脏的脂肪化、炎症和坏死的评分均明显高于对照组(P<0.05)。结论灌服酒精加以饲喂高脂饲料能成功建立酒精性肝损伤大鼠模型。
Objective To study the ideal way to establish rat model of alcoholic liver injury. Methods Thirty healthy male Sprague-Dawley rats were randomly divided into control group and model group with 15 rats in each group. The model group was given high-fat diet and fed with white spirit for four weeks. At the end of the experiment, blood was collected from the orbital venous plexus of rats and serum levels of glutamate aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), liver superoxide dismutase (SOD), malondialdehyde (MDA), hepatic glycogen content, hepatocyte apoptosis and hepatic coefficient. Results The liver index, serum TC, TG and HDL-C, liver glycogen, liver MDA and SOD in model group were significantly higher than those in control group (P <0.05) Serum ALT and AST were significantly higher than those in the control group (P <0.01). The hepatic steatosis, inflammation and necrosis scores in the model group were significantly higher than those in the control group (P <0.05). Conclusion Alcohol-fed rats fed with high-fat diet successfully established alcoholic liver injury model in rats.