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目的通过测定11种石豆兰属植物的ITS序列,为石豆兰属植物分子鉴定和遗传多样性研究提供依据。方法利用PCR法从叶片基因组DNA中克隆ITS序列,并借助生物信息学软件对其进行分析。结果 11种石豆兰属植物的ITS全长为633～645 bp,5.8 S序列长度162 bp,ITS1和ITS2序列变异位点丰富,共168个,其中信息位点95个,序列存在大量的转换、颠换和缺失;5.8 S序列较为保守,仅含6个变异位点和2个信息位点;11种石豆兰属植物的遗传距离为0.002 2～0.212 0,其中齿瓣石豆兰与斑唇石豆兰之间的遗传距离最小,亲缘关系最为接近。序列已上传至NCBI,登录号为JN619409～JN619419。结论获得了11种石豆兰属植物的rDNA ITS序列,为石豆兰属植物分子鉴定和遗传多样性研究奠定了基础。
Objective To determine the ITS sequence of 11 species of genus Styrax, and to provide the basis for molecular identification and genetic diversity of genus Styracifolium. Methods The ITS sequence was cloned by PCR from genomic DNA of leaves and analyzed by bioinformatics software. Results The ITS sequences of 11 species of genus Styrax were 633-645 bp in length and 162 bp in 5.8 S sequence. The ITS1 and ITS2 sequences were rich in variation sites with a total of 168 loci, of which there were 95 informative sites with a large number of transitions 5.8S sequences were more conservative with only 6 variation sites and 2 information sites. The genetic distances among 11 species of genus Styrax were 0.002 2 ~ 0.212 0, among which, Genetic distance is the smallest, the closest genetic relationship. The sequence has been uploaded to NCBI with accession numbers JN619409 ~ JN619419. Conclusion The rDNA ITS sequences of 11 species of genus Styrax were obtained, which laid the foundation for the molecular identification and genetic diversity of genus Styracifolium.