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目的采用PNA-FISH技术进行快速、准确的鉴定血培养中白色假丝酵母菌的方法学初索。方法模拟血培养阳性标本的制备;对所有血培养报阳的标本均进行革兰染色,初步区分报阳菌的种类;对于镜下显示为念珠菌的标本用两种方法对其进行鉴定,一种为PNA-FISH技术,另一种为传统的科马嘉念珠菌显色培养基鉴定技术,最后对这两种方法检测白色假丝酵母菌的特异度、敏感度及检测时间做对比分析。结果 PNA-FISH方法鉴定血培养中白色假丝酵母菌的特异度为100.0%,敏感度为97.5%;采用PNA-FISH技术鉴定血培养中白色假丝酵母菌的时间为(4±0.2)h,较传统真菌鉴定时间(18~24)h明显缩短。结论采用PNA-FISH技术鉴定血培养中白色假丝酵母菌特异性强,敏感度高,所需时间短,值得临床推广。
Objective To use PNA-FISH technology for rapid and accurate identification of Candida albicans in blood culture methodology. Methods The preparation of positive blood culture samples was simulated. Gram stain was performed on all the blood culture positive samples, and the types of positive bacteria were preliminarily identified. Candida samples were microscopically identified by two methods, one PNA-FISH technology for the species, and the other for the traditional identification of Candida kawachi chromogenic medium, and finally the specificity of these two methods to detect Candida albicans, sensitivity and detection time for comparative analysis. Results The PNA-FISH method was used to identify Candida albicans in blood culture with a specificity of 100.0% and a sensitivity of 97.5%. PNA-FISH was used to identify Candida albicans in blood culture for 4 ± 0.2 h , Significantly shorter than the traditional identification of fungi (18 ~ 24) h. Conclusion The identification of Candida albicans in blood culture by PNA-FISH is of high specificity, high sensitivity and short time and is worthy of clinical promotion.