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[ 目的 ]筛选能引起重症肌无力的抗乙酰胆碱受体自身抗体 ,为探讨抗体的分子结构与致病性的关系准备材料 .[方法 ]将一株抗乙酰胆碱受体上主要免疫区的单克隆抗体A7被动地转移至健康大鼠 ,根据动物发病后的临床体征、体重丧失率和全身肌肉乙酰胆碱受体损失率判定致病性 .[结果 ]大鼠在接受A7注射后 48h即已出现严重的实验性自身免疫性重症肌无力 ,体重丧失率和全身肌肉乙酰胆碱受体损失率分别为 (6 .8± 1.7) %和 (38.4±7.2 ) % .[结论 ]A7是一株致病性很强的抗乙酰胆碱受体自身抗体
[Objective] To screen anti-acetylcholine receptor autoantibodies that can cause myasthenia gravis, and to prepare the materials for exploring the relationship between the molecular structure and pathogenicity of the antibodies. [Method] A monoclonal antibody against the main immune domain of acetylcholine receptor A7 was passively transferred to healthy rats, and the pathogenicity was judged according to the clinical signs, the rate of weight loss and the rate of loss of body muscle acetylcholine receptor after the onset of the disease. [Results] A serious experiment occurred 48h after the rats received A7 injection The autoimmune myasthenia gravis, body weight loss rate and total body muscle acetylcholine receptor loss rate were (6.8 ± 1.7)% and (38.4 ± 7.2)%, respectively. [Conclusion] A7 is a highly pathogenic Anti-acetylcholine receptor autoantibodies