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目的探索超顺磁性氧化铁纳米粒子(SPIO)标记神经干细胞体的方法及其检测手段,研究标记细胞移植后在活体上磁共振信号的改变。方法分离培养新生鼠皮层神经干细胞,使用SPIO和多聚赖氨酸联合标记神经干细胞;将标记后的神经干细胞移植入Wistar大鼠脑内,应用不同的扫描序列对脑内移植的神经干细胞进行示踪。结果 体外标记的神经干细胞普鲁士蓝染色见细胞浆内有许多蓝染的铁颗粒,电镜检查表明SPIO颗粒存在于标记细胞的吞饮小泡及细胞基质内,标记后的细胞可正常分化。脑内移植的标记细胞在磁共振上呈明显的低信号改变,以GRE T2序列信号改变最为明显。结论超顺磁性氧化铁粒子可以用来标记神经干细胞,且对细胞增殖、分化无影响,SPIO颗粒存在于标记细胞的吞饮小泡及细胞基质内。标记后体内移植的神经干细胞可以在MR上产生明显的低信号改变。
OBJECTIVE: To investigate the method of SPIO-labeled neural stem cell and its detection method, and to study the change of magnetic resonance signals in vivo after labeled cell transplantation. Methods Neural stem cells of neonatal rat cortical neurons were isolated and cultured, SPIO and poly-L-lysine were used to label neural stem cells. The labeled neural stem cells were transplanted into the brain of Wistar rats. Different kinds of neural stem cells trace. Results Prussian blue staining in vitro showed that there were many blue-stained iron particles in the cytoplasm. Electron microscopy showed that the SPIO particles existed in the swallowed vesicles and the cell matrix of labeled cells, and the labeled cells could differentiate normally. Marked intracerebral transplantation of labeled cells in the magnetic resonance was significantly lower signal changes to GRE T2 sequence signal changes the most obvious. Conclusion Superparamagnetic iron oxide particles can be used to label neural stem cells, and have no effect on cell proliferation and differentiation. SPIO particles are present in the phagocytic vesicles and the cell matrix of labeled cells. Transplantation of labeled neural stem cells in vivo can produce significant low signal changes on MR.