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目的 探讨铁剥夺对HL 6 0细胞凋亡及对化疗药物诱导HL 6 0细胞凋亡的影响。方法 HL 6 0细胞与不同浓度的铁螯合剂———去铁胺 (DFO)、DFO加化疗药物、化疗药物、DFO加化疗药物及三氯化铁、三氯化铁共同培养 6h、12h、2 4h、48h。通过测定细胞活力 ,观察细胞形态学变化 ,应用流式细胞仪检测和DNA凝胶电泳等方法观察细胞凋亡 ;通过亲和免疫组化方法检测c myc基因表达 ,从而确定DFO及DFO与化疗药物联合应用对HL 6 0细胞的作用。结果 DFO单用可降低HL 6 0细胞活力、抑制HL 6 0细胞增殖、诱导HL 6 0凋亡 ,并可使c myc基因表达增加 ,其作用强度随培养时间延长及DFO浓度增加而增加。DFO与化疗药物联合时 ,可增加化疗药物诱导HL 6 0细胞凋亡的作用。该作用可被等摩尔的三氯化铁抵消。结论 铁剥夺可影响HL 6 0细胞DNA的合成 ,诱导其凋亡 ,并提高HL 6 0细胞对化疗药物的敏感性。因此 ,铁剥夺可作为临床上白血病治疗或辅助治疗的方法之一 ,不适当补铁或升高血红蛋白将影响化疗疗效。
Objective To investigate the effect of iron deprivation on the apoptosis of HL 60 cells and the effect of chemotherapy drugs on apoptosis of HL 60 cells. Methods HL 60 cells were incubated with different concentrations of iron chelators—deferoxamine (DFO), DFO plus chemotherapeutic drugs, chemotherapeutic drugs, DFO plus chemotherapeutic drugs, ferric chloride, and ferric chloride for 6 h and 12 h. 2 4h, 48h. The cell viability was observed and the morphological changes were observed. The cell apoptosis was observed by flow cytometry and DNA gel electrophoresis. The expression of c myc gene was detected by affinity immunohistochemistry to determine DFO and DFO and chemotherapeutic drugs. Combined use of effects on HL 60 cells. RESULTS: DFO alone could reduce the viability of HL 60 cells, inhibit the proliferation of HL 60 cells, induce apoptosis of HL 60, and increase the expression of c myc gene. Its intensity of action increased with the increase of culture time and increase of DFO concentration. Combination of DFO and chemotherapeutic agents can increase the effect of chemotherapeutic agents in inducing apoptosis of HL 60 cells. This effect can be offset by an equimolar amount of ferric chloride. Conclusion Iron deprivation can affect DNA synthesis in HL 60 cells, induce apoptosis, and increase the sensitivity of HL 60 cells to chemotherapeutic drugs. Therefore, iron deprivation can be used as one of the methods for clinical leukemia treatment or adjuvant therapy. Improper iron supplementation or elevated hemoglobin will affect the efficacy of chemotherapy.