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目的观察14-3-3ζ和β连接素(β-catenin)在人T1期非小细胞肺癌(NSCLC)中的表达,探讨两者与非小细胞肺癌发展、侵袭和转移的关系。方法采用免疫组织化学、免疫荧光双标、激光扫描共焦显微技术和Western blotting,检测NSCLC及正常肺组织中14-3-3ζ和β-catenin蛋白的表达。结果与正常肺组织相比,14-3-3ζ在NSCLC中的表达显著增强,且表达与肺癌的分化程度和淋巴结转移有关,而与病理学分型无关。β-catenin在NSCLC中的表达与正常肺组织相比减弱,且其表达与肺癌的分化程度呈正相关,与淋巴结转移呈负相关,而与肺癌病理学分型无关。结论14-3-3ζ和β-catenin与T1期NSCLC发展、侵袭和转移密切相关。14-3-3ζ高表达可能促进了NSCLC的发展与转移,因此14-3-3ζ可以作为非小细胞肺癌又一个有效的治疗靶点。
Objective To observe the expression of 14-3-3ζ and β-catenin in human T1 non-small cell lung cancer (NSCLC) and to explore their relationship with the development, invasion and metastasis of non-small cell lung cancer. Methods The expressions of 14-3-3ζ and β-catenin protein in NSCLC and normal lung tissue were detected by immunohistochemistry, double immunofluorescence staining, laser scanning confocal microscopy and Western blotting. Results Compared with normal lung tissue, the expression of 14-3-3ζ in NSCLC was significantly increased. The expression of 14-3-3ζ was correlated with the differentiation and lymph node metastasis of lung cancer, but not with the pathological type. The expression of β-catenin in NSCLC was lower than that in normal lung tissue, and the expression of β-catenin was positively correlated with the differentiation degree of lung cancer and negatively correlated with lymph node metastasis, but not with the pathological type of lung cancer. Conclusion 14-3-3ζ and β-catenin are closely related to the development, invasion and metastasis of T1-stage NSCLC. High expression of 14-3-3ζ may promote the development and metastasis of NSCLC, so 14-3-3ζ can be used as another effective therapeutic target for non-small cell lung cancer.