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目的探讨糖尿病自身抗体联合检测的临床意义。方法对2008年5月至2009年9月在沈阳军区总医院内分泌科就诊的1500例糖尿病患者行酶联免疫印迹法检测多种糖尿病自身抗体-谷氨酸脱羧酶抗体(GADA)、胰岛细胞抗体(ICA)、胰岛素自身抗体(IAA)、酪氨酸磷酸酶抗体(IA-2A),电化学发光法定量测定75 g葡萄糖及100 g馒头餐负荷后的胰岛素-C肽释放曲线,多因素分析糖尿病自身抗体的表达。结果联合抗体检测较单一抗体检测阳性率明显提高。结论糖尿病自身抗体联合检测敏感性强,可明显提高阳性检出率,为糖尿病的分型诊断及治疗提供临床依据。
Objective To investigate the clinical significance of combined detection of autoantibodies in diabetes mellitus. Methods A total of 1 500 diabetic patients treated in Department of Endocrinology of General Hospital of Shenyang Military Command from May 2008 to September 2009 were detected by enzyme-linked immunosorbent assay (ELISA) for a variety of autoantibodies to diabetes - glutamic acid decarboxylase antibody (GADA), islet cell antibody (ICA), insulin autoantibodies (IAA), and tyrosine phosphatase antibody (IA-2A). The levels of insulin-C peptide after 75 g of glucose and 100 g of steamed bread were measured by electrochemiluminescence. Multivariate analysis Diabetes autoantibody expression. Results The detection rate of combined antibody was significantly higher than that of single antibody. Conclusion The combined detection of diabetes autoantibodies is highly sensitive and can significantly improve the positive detection rate, providing a clinical basis for the diagnosis and treatment of diabetes.