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目的研究人粥样硬化病变主动脉中丙二醛（ＭＤＡ）和４羟基壬烯醛（ＨＮＥ）修饰载脂蛋白Ｂ（ａｐｏＢ）的分布特点、含量和理化特性，并与脂蛋白（ａ）［Ｌｐ（ａ）］和ａｐｏＢ的分布进行比较。方法应用免疫组织化学、电镜、免疫电镜以及生物化学等方法进行定性或定量分析。结果ＭＤＡ和ＨＮＥ－ａｐｏＢ在细胞外基质中的分布与Ｌｐ（ａ）和ａｐｏＢ一致，但在泡沫细胞内则有不同。ＭＤＡ－ａｐｏＢ和ＨＮＥ－ａｐｏＢ在泡沫细胞内呈环状、孔状分布，与蜡样色素相似。病变动脉内膜低密度脂蛋白的超微结构、化学成分及电泳行为均与体外修饰脂蛋白相似，其醛类修饰ａｐｏＢ含量明显高于无病变者。结论脂蛋白的氧化性修饰是动脉粥样硬化发生的必要条件。
Objective To investigate the distribution, content and physico-chemical properties of malondialdehyde (MDA) and 4-hydroxynonenal (HNE) -modified apolipoprotein B in human aortic atherosclerotic lesions and compare them with lipoprotein (a) Lp (a)] and apoB. Methods Immunohistochemistry, electron microscopy, immunoelectron microscopy and biochemical methods such as qualitative or quantitative analysis. Results The distribution of MDA and HNE-apoB in the extracellular matrix was consistent with Lp (a) and apoB, but different in foam cells. MDA-apoB and HNE-apoB in foam cells were ring-shaped, pore-like distribution, and waxy pigment similar. The ultrastructure, chemical composition and electrophoretic behavior of arterial LDL were similar to that of modified lipoprotein in vitro, and the contents of apoB in aldehyde-modified apoB were significantly higher than those without lesions. Conclusion Oxidative modification of lipoprotein is a necessary condition for the development of atherosclerosis.