啤酒花根癌病在我国普遍发生。自1981年春,在石河子地区发现此病以来,伊犁、阿克苏、库尔勒等地区也相继发现。在淅江的杭州、肖山、山东的泰安、青岛等地亦采到病瘤。1982—1984年将新疆、山东、浙江等地采回的病瘤和病土。经直接和间接分离到61个菌株,通过培养性状,革兰氏染色反应、鞭毛染色等常规鉴定,并将菌株回接到啤酒花根颈部,均产生病瘤,接种在番茄茎部经过10—25天均产生瘿瘤,确定该菌为根癌土壤杆菌[Agrobacterium tumefaciens(smith＆Townsend)Conn]。根癌土壤杆菌分三个不同生物型,从不同地区分离到的菌株,用3—酮苷乳糖、柠檬酸铁铵测定为阳性反应,石蕊牛乳为碱性反应,以及对糖、醇、盐的反应和特殊培养基生长等表明,啤酒花根癌土壤杆菌为生物型1,现尚未发现有另外两个型。 Hops cancer is common in our country. Since the spring of 1981, the disease has been found in the Shihezi area, Ili, Aksu, Korla and other areas have also been found. In the Han River in Hangzhou, Xiao Shan, Tai’an, Shandong, Qingdao and other places also taken to the tumor. 1982-1984 Xinjiang, Shandong, Zhejiang and other places to recover the disease and disease soil. 61 strains were directly and indirectly isolated and identified by culture traits, Gram stain reaction and flagella staining, and the strains were returned to the hops and roots of the hops. All of them produced tumors, which were inoculated in 10- Tumor was produced on average 25 days and Agrobacterium tumefaciens (smith & Townsend) Conn was identified. Agrobacterium tumefaciens is divided into three different biotypes, isolated from different regions of the strain, with 3-keto-lactose, ferric ammonium citrate as a positive reaction, litmus milk as a basic reaction, as well as sugar, alcohol, salt The reaction and the growth of special media showed that A. tumefaciens was biotype 1, and no other two types were found.