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目的:建立测定妇宁康软胶囊中淫羊藿苷含量的HPLC法。方法:采用十八烷基硅烷键合硅胶柱,流动相为乙腈-水-冰乙酸(27:75:0.6),检测波长为270nm,柱温为25℃,流速为1.0mL·min-1,对其进行了含量测定。结果:淫羊藿苷在3-18μg·mL-1内质量浓度与峰面积呈良好的线性关系,线性回归方程为A=7.69×105C+792.5,r=0.9999,平均回收率为100.3%(RSD=2.27%)。结论:该法准确度高、专属性好,可用于妇宁康软胶囊的含量测定。
Objective: To establish HPLC method for determination of icariin content in Fu Ning Kang soft capsules. Methods: The octadecylsilane bonded silica gel column was used. The mobile phase was acetonitrile-water-glacial acetic acid (27:75:0.6), the detection wavelength was 270 nm, the column temperature was 25°C, and the flow rate was 1.0 mL·min-1. Its content was determined. RESULTS: There was a good linear relationship between icariin mass concentration and peak area in 3-18 μg·mL-1. The linear regression equation was A=7.69×105C+792.5, r=0.9999, and the average recovery was 100.3% (RSD =2.27%). Conclusion: This method has high accuracy and specificity and can be used for the determination of Fu Ning Kang Capsule.