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目的建立并比较两种IgA肾病大鼠模型。方法 36只雄性SD大鼠随机均分为三组:A组采用口服免疫原+皮下注射四氯化碳(CCl4)和免疫佐剂+静脉注射葡萄球菌肠毒素的方法建立IgA肾病模型;B组采用口服免疫原+皮下注射CCl4+静脉注射脂多糖(LPS)的方法建模;C组为正常对照组。分别在建模第6、8和10周末观察各组24-h尿蛋白定量,第10周末处死大鼠后取动脉血检测肝、肾功能,检查肾组织病理和IgA免疫荧光。结果与C组相比,A、B组第8和10周末24-h尿蛋白定量增加(P<0.05),第10周末血尿素氮、血清肌酐水平升高,白蛋白水平降低(P<0.01),且B组较A组变化更明显(P<0.05或P<0.01)。与A组相比,B组IgA免疫荧光强度增加(P<0.01),肾脏病变加重。结论采用口服免疫原+皮下注射CCl4+静脉注射LPS的方法建立IgA肾病大鼠模型的效果更好。
Objective To establish and compare two rat models of IgA nephropathy. Methods Thirty-six male Sprague-Dawley rats were randomly divided into three groups: group A, IgA nephropathy model was established by oral immunization with subcutaneous injection of carbon tetrachloride (CCl4) and immune adjuvant + intravenous injection of staphylococcal enterotoxin; group B The oral immunogen + subcutaneous injection of CCl4 + intravenous injection of lipopolysaccharide (LPS) modeling; C group as the normal control group. The levels of 24-h urinary protein in each group were observed at the 6th, the 8th and the 10th week of modeling respectively. After the rats were sacrificed at the end of the 10th week, the blood and blood were collected to detect the function of the liver and the kidneys. The renal pathology and IgA immunofluorescence were examined. Results Compared with group C, 24-h urinary protein in groups A and B increased quantitatively at the end of the 8th and 10th weeks (P <0.05). At the end of the 10th week, blood urea nitrogen, serum creatinine level and albumin level decreased (P <0.01 ), And the change of group B was more obvious than that of group A (P <0.05 or P <0.01). Compared with group A, immunostaining of IgA in group B increased (P <0.01), and the pathological changes of kidney increased. Conclusion The oral immunogen + subcutaneous injection of CCl4 + intravenous injection of LPS method to establish a rat model of IgA nephropathy better.