论文部分内容阅读
目的探讨新型羧基化半抗原直接包被酶联免疫吸附实验(ELISA)法测定水中残留阿特拉津的可行性。方法使用烷基化试剂3-氨基丙基三乙氧基甲硅烷(APTES)在聚苯乙烯表面构建活性的伯胺基,羧基化阿特拉津经活化后进攻伯胺基形成稳定的酰胺键从而完成羧基化半抗原直接偶联。羧基化半抗原直接包被ELISA法测定水中阿特拉津残留。结果该方法检出限为0.68 ng/mL。该抗体与西玛津交叉反应率为24%,其他类似物交叉反应率均小于0.1%,特异性较高。在实际样本分析中具有较高的准确度(添加回收率94.0%~112.0%)和稳定性(相对标准偏差2.72%~3.53%)。结论该方法用于检测水中残留阿特拉津,方法简便,结果可靠。
Objective To investigate the feasibility of direct determination of residual atrazine in water by enzyme-linked immunosorbent assay (ELISA) with novel carboxylated haptens. The method uses alkylating reagent 3-aminopropyltriethoxysilane (APTES) to construct active primary amine group on the surface of polystyrene, and the activated carboxylated atrazine is activated to attack the primary amine group to form stable amide bond Thus completing the direct coupling of the carboxylated hapten. The carboxylated hapten was directly coated by ELISA for the determination of atrazine residues in water. Results The detection limit of this method was 0.68 ng / mL. The antibody cross-reactivity with simazine 24%, other analog cross-reactivity rates were less than 0.1%, high specificity. In the actual sample analysis with high accuracy (addition recovery 94.0% ~ 112.0%) and stability (relative standard deviation 2.72% ~ 3.53%). Conclusion The method is suitable for the determination of residual atrazine in water. The method is simple and reliable.