目的探讨一氧化碳（ＣＯ）等在小儿哮喘发病机制中的作用及意义。方法应用Ｃｈａｌｍｅｒｓ血红蛋白结合及联二亚硫酸盐还原法检测了２２名正常儿童、２６例支气管哮喘患儿血浆ＣＯ水平，同时测定了一氧化氮（ＮＯ）和肿瘤坏死因子α（ＴＮＦα）水平，并在动物模型上进行验证。结果哮喘患儿急性发作期血浆ＣＯ、ＮＯ２／ＮＯ３和ＴＮＦα水平与对照组比较，ｔ值分别为８４２１，３０８８，１５４３２，（Ｐ均＜０．０１），缓解期ＣＯ和ＮＯ２／ＮＯ３水平恢复正常（Ｐ均＞０．０５），而ＴＮＦα水平与对照组比较差异有显著性（Ｐ＜０．０１）。哮喘患儿血浆ＣＯ与ＮＯ２／ＮＯ３水平呈显著正相关（ｒ＝０．４７６，Ｐ＜０．０５），而与ＴＮＦα相关不显著（ｒ＝０．２６８，Ｐ＞０．０５）。过敏性哮喘豚鼠血浆ＣＯ和ＴＮＦα水平与对照组比较，ｔ值分别为３６９４，７４５４，（Ｐ均＜０．０１），而ＮＯ２／ＮＯ３水平变化差异无显著性（Ｐ＞０．０５）。过敏性哮喘豚鼠支气管肺泡灌洗液（ＢＡＬＦ）中ＣＯ、ＮＯ２／ＮＯ３和ＴＮＦα水平的ｔ值分别为３０４９，５６０９，５９５５，（Ｐ＜０．０５～００１）。糖皮质激素组血浆及ＢＡＬＦ中? Objective To investigate the role and significance of carbon monoxide (CO) in the pathogenesis of asthma in children. Methods Plasma levels of CO in 22 normal children and 26 children with bronchial asthma were detected by Chalmers hemoglobin binding and dithionite reduction. The levels of nitric oxide (NO) and tumor necrosis factor α (TNFα) , And validated on animal models. Results Compared with the control group, the levels of CO, NO2 / NO3 and TNFα in acute exacerbation of asthmatic children were 8421, 3088 and 15432 (P <0.01 respectively) ), And the levels of CO and NO2 / NO3 returned to normal in remission (all P> 0.05), while the levels of TNFα were significantly different from those in control group (P <0.01). There was a significant positive correlation between plasma CO and NO2 / NO3 in asthmatic children (r = 0.476, P <0.05), but not correlated with TNFα (r = 0.268, P> 0 .05). The levels of plasma CO and TNFα in guinea pigs with allergic asthma were significantly higher than those in the control group (t = 3694, 7454, respectively, P <0.01), while there was no significant difference in NO2 / NO3 levels (P> 0.05). The t values ​​of CO, NO2 / NO3 and TNFα in bronchoalveolar lavage fluid (BALF) of allergic asthma in guinea pigs were 3049, 5609, 5955, respectively (P <0.05 ~ 001). Glucocorticoid plasma and BALF?