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目的检测复发性生殖器疱疹(RGH)患者外周血中T细胞亚群和共刺激分子的表达,探讨其在RGH免疫中的作用。方法用免疫荧光技术和流式细胞仪检测RGH及对照组血中T细胞亚群和共刺激分子(CD28,CD25,Fas,FasL,4-1BB)的水平。结果 RGH组CD3+,CD4+/CD8+,CD4+CD28+细胞百分数(59.48±10.9,0.89±0.20,27.69±7.34)显著低于健康对照组(69.79+5.63,1.14+0.39,33.68±7.32),差异均有统计学意义(P<0.05);CD4+CD28-,CD25+细胞百分数及Fas(6.55±4.46,15.81±8.62,27.58±3.10)显著高于健康对照(1.93±1.36,7.13±5.13,23.11±5.63),差异均有统计学意义(P<0.01,P<0.05)。结论 RGH外周血CD3+,CD4+/CD8+下降,CD4+CD28+低表达,及CD25+和Fas高表达,可能是RGH复发的免疫障碍环节之一。
Objective To detect the expression of T lymphocyte subsets and costimulatory molecules in peripheral blood of patients with recurrent genital herpes (RGH) and to explore its role in RGH immunization. Methods The level of T lymphocyte subsets and costimulatory molecules (CD28, CD25, Fas, FasL, 4-1BB) in RGH and control groups were detected by immunofluorescence and flow cytometry. Results The percentages of CD3 +, CD4 + / CD8 + and CD4 + CD28 + cells in RGH group were significantly lower than those in healthy control group (69.79 + 5.63, 1.14 + 0.39, 33.68 ± 7.32) (59.48 ± 10.9,0.89 ± 0.20,27.69 ± 7.34) The percentage of CD4 + CD28-, CD25 + cells and Fas (6.55 ± 4.46, 15.81 ± 8.62, 27.58 ± 3.10) were significantly higher than those of healthy controls (1.93 ± 1.36, 7.13 ± 5.13, 23.11 ± 5.63) , The differences were statistically significant (P <0.01, P <0.05). Conclusions The decrease of CD3 +, CD4 + / CD8 +, the low expression of CD4 + CD28 + and the high expression of CD25 + and Fas in the peripheral blood of RGH may be one of the immunological barriers of RGH recurrence.