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目的探讨胆管癌细胞ICAM-1和IGF-Ⅱ的表达与胆管癌转移、预后关系。方法常规石蜡切片,用鼠抗人IGF-Ⅱ、ICAM-1单克隆抗体免疫组化法,光学显微镜观察胆管癌细胞阳性染色。结果(1)胆管癌IGF-Ⅱ阳性表达率为52.9%(36/68),主要在胞浆和胞核表达;I-CAM-1阳性表达率为61.8%(42/68),主要在胞浆和胞膜表达。(2)ICAM-1和IGF-Ⅱ在分化好的胆管癌表达率低于分化差的胆管癌。(3)IGF-Ⅱ高表达,肿瘤组织中内皮细胞丰富,与血管生成密切相关。ICAM-1和IGF-Ⅱ高表达,存活时间短,易发生转移(88.9%)。结论胆管癌ICAM-1和IGF-Ⅱ共同高表达,使肿瘤细胞更易于从肿瘤组织中脱落、迁移,使胆管癌细胞易于侵袭周边组织,增加远处转移机会。
Objective To investigate the relationship between the expression of ICAM-1 and IGF-Ⅱ and the metastasis and prognosis of cholangiocarcinoma. Methods The normal paraffin section was used to observe the positive staining of cholangiocarcinoma cells by immunohistochemistry with mouse anti-human IGF-Ⅱ and ICAM-1 monoclonal antibody. Results (1) The positive expression rate of IGF-Ⅱ in cholangiocarcinoma was 52.9% (36/68), which was mainly expressed in cytoplasm and nucleus. The positive expression rate of I-CAM-1 was 61.8% (42/68) Plasma and membrane expression. (2) The expression rate of ICAM-1 and IGF-Ⅱ in differentiated cholangiocarcinoma is lower than that in poorly differentiated cholangiocarcinoma. (3) High expression of IGF-Ⅱ, endothelial cell rich in tumor tissue, and angiogenesis are closely related. High expression of ICAM-1 and IGF-Ⅱ, survival time is short, prone to metastasis (88.9%). Conclusions The common expression of ICAM-1 and IGF-Ⅱ in cholangiocarcinoma makes the tumor cells more easily shed and migrate from the tumor tissue, making it easier for the cholangiocarcinoma cells to invade the surrounding tissues and increase the chance of distant metastasis.