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用LaCl3,CeCl3处理菠菜后证实La3+,Ce3+处理可诱导菠菜体内形成Rubisco和Rubisco活化酶的复合体.从LaCl3,CeCl3处理的菠菜体内纯化得到的蛋白其SDS-PAGE电泳分析证明除具有Rubisco的大小亚基(55,14.4 kd)之外,在邻近大亚基处还具有Rubisco活化酶的45和41 kd的两个大亚基;非变性PAGE电泳分析证明除具有Rubisco的一条带(560 kd)以外,在距Rubisco大约2倍距离处还有一条分子量在1100 kd左右的带,推测为Rubisco和Rubisco活化酶的复合体.La3+,Ce3+处理的酶活性分别是对照纯Rubisco的1.8和2.8倍,吸收光谱和荧光发射光谱强度也明显高于对照的纯Rubisco,总巯基数均比对照纯Rubisco每摩尔多36-39个,表面巯基数分别多14-25个,CD光谱计算表明La3+,Ce3+处理的酶二级结构与对照的有较大差异.体内酶活性测定表明La3+,Ce3+处理的为对照的1.5和1.9倍.
Treatment of spinach with LaCl3 and CeCl3 confirmed that the complex of Rubisco and Rubisco activase could be induced by La3 + and Ce3 + treatment.The protein purified from LaCl3 and CeCl3-treated spinach was analyzed by SDS-PAGE electrophoresis, In addition to the subunits (55,14.4 kd), there are two large subunits of Rubisco activase at 45 and 41 kd adjacent to the large subunit; non-denaturing PAGE electrophoresis analysis shows that in addition to a band with Rubisco (560 kd) In addition, a band with a molecular weight of about 1100 kd at about two times the distance from Rubisco was presumed to be a complex of Rubisco and Rubisco activase. The enzymatic activities of La3 + and Ce3 + treatments were 1.8 and 2.8 times that of pure Rubisco, respectively, The intensity of absorption and fluorescence emission was also significantly higher than that of the control. The total number of mercapto groups was 36-39 more than that of the control Rubisco and the number of mercapto groups was 14-25 respectively. CD spectra showed that La3 + and Ce3 + Of the secondary structure of the enzyme and the control there is a big difference in vivo enzyme activity determination showed that La3 +, Ce3 treatment 1.5 and 1.9 times the control.