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目的:研究荧光增白剂CBS(flourescent brightener CBS,CBS)对红色毛癣菌的抗菌作用,以及联合使用酮康唑和特比萘芬的增效作用。方法:采用滤纸片扩散法,探究CBS对红色毛癣菌的单一抗菌作用;采用MTT法探究CBS联合酮康唑、特比萘芬对红色毛癣菌的协同抗菌作用;然后采用均匀设计方法结合SPSS软件建立模拟方程,并通过验证试验确定最优配方;用豚鼠构建红色毛癣菌感染的动物模型,评价最优配方的实际药用效果。结果:酮康唑、特比萘芬联合CBS均对其存在协同作用。均匀设计得到最优配方是CBS浓度为1.861 mg·mL~(-1),酮康唑浓度为1.5μg·mL~(-1),特比萘芬浓度为0.175μg·mL~(-1),SDS浓度为0.077 4 mg·mL~(-1),氮酮浓度为6 mg·mL~(-1)时,理论最大抑制率为75.34%。进行动物试验表明联合药物比单一药物平均缩短了6 d的治疗时间。结论:CBS对红色毛癣菌有抗菌作用,联合酮康唑和特比萘芬对红色毛癣菌具有协同增效作用。
Objective: To study the antibacterial effect of flourescent brightener CBS (CBS) on Trichophyton rubrum and the synergistic effects of ketoconazole and terbinafine. Methods: A single antimicrobial effect of CBS against Trichophyton rubrum was explored by the filter paper diffusion method. The synergistic antimicrobial effect of CBS combined with ketoconazole and terbinafine on Trichophyton rubrum was investigated by MTT assay. Then, SPSS software was used to establish the simulation equation, and the optimal formulation was confirmed through the validation test. The animal model infected with Trichophyton rubrum was constructed by guinea pig to evaluate the practical effect of the optimal formula. Results: Both ketoconazole and terbinafine combined with CBS had a synergistic effect on them. The optimal formulation for uniform design was CBS concentration of 1.861 mg · mL -1, ketoconazole concentration of 1.5 μg · mL -1 and terbinafine concentration of 0.175 μg · mL -1, , The maximum inhibition rate of SDS was 75.34% when the concentration of SDS was 0.077 4 mg · mL -1 and the concentration of azone was 6 mg · mL -1. Animal studies showed that combination drugs shortened the 6-day treatment time on average compared to single drugs. CONCLUSION: CBS has antibacterial activity against Trichophyton rubrum and synergism with Trichophyton rubrum via ketoconazole and terbinafine.