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目的:研究凹土玉米芯垫料对细胞毒性作用。方法:将凹土玉米芯,玉米秸,普通刨花垫料物质的丙酮提取物分别加到H22-H2D8细胞中培养,测定每个培养孔细胞的总蛋白量,计算细胞的半数致死量(LD50);MTT法检测不同垫料丙酮提取物对H22-H2D8细胞增殖率的影响,用6级毒性分类法进行毒性分级。结果:普通刨花垫料LD50<3 g.L-1,玉米秸垫料LD50<20 g.L-1,凹土玉米芯垫料LD50>40 g.L-1。在垫料提取物浓度为10 g.L-1时,普通刨花的细胞毒性5级,玉米秸的细胞毒性为2级,凹土玉米芯的细胞毒性为1级;凹土玉米芯垫料组细胞增殖率显著高于玉米秸组和普通刨花组(P<0.01)。结论:普通刨花的细胞毒性最大,凹土玉米芯的细胞毒性最小,玉米秸、普通刨花垫料物质均有较大的细胞毒性,其毒性显著高于凹土玉米芯。
Objective: To study the cytotoxic effect of sunken corncob litter. Methods: The acetone extract of cot corn cob, corn stalks and ordinary wood litter were respectively added into H22-H2D8 cells for culture. The total protein of each well was measured, and the half-lethal dose (LD50) MTT assay was used to examine the effect of different litter acetone extract on proliferation rate of H22-H2D8 cells. Results: The common wood shaving litter LD50 <3 g.L-1, the corn stalk litter LD50 <20 g.L-1, the concave soil corncob litter LD50> 40 g.L-1. When the litter extract concentration was 10 gL-1, the cytotoxicity of common wood shavings was grade 5, the cytotoxicity of corn stalk was level 2, and the cytotoxicity of attapulgite corncob was grade 1. The cell proliferation The rate was significantly higher than corn stalk group and common wood shaving group (P <0.01). CONCLUSION: The common wood shavings have the highest cytotoxicity, the lowest toxicity of attapulgite corncobs, and the corn stalks and common wood shavings materials have greater cytotoxicity, and their toxicity is significantly higher than that of attapulgite core.