Monte Carlo simulation of light propagation in turbid medium has been studied for years. A number of software packages have been developed to handle with such issue. However, it is hard to compare these simulation packages, especially for tissues with complex heterogeneous structures. Here, we first designed a group of mesh datasets generated by Iso2Mesh software, and used them to cross-validate the accuracy and to evaluate the performance of four Monte Carlobased simulation packages, including Monte Carlo model of steady-state light transport in multilayered tissues (MCML), tetrahedron-based inhomogeneous Monte Carlo optical simulator (TIMOS), Molecular Optical Simulation Environment (MOSE), and Mesh-based Monte Carlo (MMC). The performance of each package was evaluated based on the designed mesh datasets. The merits and demerits of each package were also discussed. Comparative results showed that the TIMOS package provided the best performance, which proved to be a reliable, e±cient, and stable MC simulation package for users.