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目的 探讨p16基因改变在肾细胞癌发生发展中的作用及临床意义。方法 采用聚合酶链反应 (PCR)加单链构象多态性分析 (SSCP)、多重对照聚合酶链反应 (multiplex PCR)、双重对照PCR反应及链霉菌亲和物素蛋白 生物素酶标免疫组织化学 (S P法 )检测 42例肾细胞癌组织、18例远离肿瘤的正常肾脏及 10例正常肾脏组织中p16基因异常及 p16蛋白的表达情况。 结果 p16基因改变在肾癌 (3 0 .95 % )及正常肾脏组织 (3 .5 1% )差异有非常显著性 (P <0 .0 1) ,其蛋白表达在肾癌 (5 7.14 % )及正常肾脏组织 (89.2 9% )差异亦有显著性 (P <0 .0 0 5 ) ,p16基因改变及蛋白表达与肾细胞癌组织学类型无关 ,p16蛋白表达与病理分级、临床分期相关。结论 p16基因失活在肾癌的发生发展中起着重要作用 ,其作用是通过不同的失活机制引起的 p16蛋白表达缺如或功能丧失而实现 ;p16基因及蛋白的检测可作为肾癌的辅助诊断、判断恶性程度参考指标。
Objective To investigate the role of p16 gene in the development of renal cell carcinoma and its clinical significance. Methods Polymerase chain reaction (PCR) plus single strand conformation polymorphism analysis (SSCP), multiplex PCR, double-control PCR and Streptomyces avidin biotin-labeled immunohistochemistry The abnormalities of p16 gene and the expression of p16 protein in 42 cases of renal cell carcinoma, 18 cases of normal kidney away from tumor and 10 cases of normal renal tissue were detected by SP method. Results There was a significant difference in p16 gene expression between renal cell carcinoma (3.95%) and normal renal tissue (3.51%) (P <0.01) (89.29%) (P <0.05). There was no correlation between the expression of p16 gene and the histological type of renal cell carcinoma. The expression of p16 protein was correlated with pathological grade and clinical stage. Conclusion Inactivation of p16 gene plays an important role in the development and progression of renal cell carcinoma. Its function is through lack of p16 protein expression or loss of function caused by different inactivation mechanisms. The detection of p16 gene and protein can be used as a marker of renal cell carcinoma Auxiliary diagnosis, to determine the degree of malignancy reference indicators.