目的运用水压法复制HBV小鼠急性模型,用于研究双表达siRNA在小鼠体内对HBV复制的抑制作用。方法使用HBV质粒DNA对BALB/c小鼠进行尾静脉大剂量快速推注,获得急性HBV体内表达模型。同时使用相同方法对推注后的小鼠进行RNAi干预。对不同处理组动物的HBsAg、HBeAg、HBcAg和HBV DNA进行ELISA、免疫组织化学和实时定量RT-PCR检测。结果水压法复制HBV小鼠急性模型与HBV DNA感染剂量无明显相关。与单表达siRNA相比较,双表达siRNA对HBV抗原表达抑制较为明显。结论双表达siRNA不失为一种体内抑制HBV感染的一种策略。 OBJECTIVE: To use hydrostatic method to replicate the acute murine model of HBV in order to study the inhibitory effect of double-siRNA on HBV replication in mice. Methods BALB / c mice were rapidly bolus injected with HBV plasmid DNA to obtain acute HBV in vivo model. The same method was used to perform RNAi intervention on the mice after bolus injection. The HBsAg, HBeAg, HBcAg and HBV DNA of different treatment groups were tested by ELISA, immunohistochemistry and real-time quantitative RT-PCR. Results There was no significant correlation between the acute model of hepatitis B virus replication and the dose of HBV DNA. Compared with the single expression of siRNA, double expression of siRNA on HBV antigen expression was more obvious. Conclusion Double expression of siRNA is a strategy of inhibiting HBV infection in vivo.