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目的 探讨抗肺癌单抗 5F11与多柔比星 (DXR)的复合物 5F11 DXR对肺癌细胞的导向治疗作用及其对一种耐药肺癌细胞系的耐药逆转作用。方法 应用戊二醛法制备 5F11 DXR ,通过集落形成试验、染料排斥试验来评价其对敏感靶细胞A2、耐药靶细胞 80 1 D和 80 1 DDXR以及非靶细胞艾氏腹水癌细胞的杀伤作用。结果 多种检测方法均显示 ,在DXR含量为 0 .4μg/ml时 ,5F11 DXR对A2、80 1 D以及 80 1 DDXR细胞的杀伤作用显著高于DXR单药 (P <0 .0 5 ) ;尤其是对敏感细胞株A2细胞 ,在更低浓度 (0 .0 4μg/ml)即可表现出此种差异。但对非靶细胞艾氏腹水癌细胞 ,两者的杀伤作用无显著性差异 (P >0 .0 5 )。结论 5F11 DXR对敏感靶细胞的杀伤作用较DXR明显增强 ,并可有效逆转耐药靶细胞对DXR的多药耐药性。两者对非靶细胞的杀伤作用无显著性差异。
Objective To investigate the role of 5F11 DXR, a complex of anti-lung cancer monoclonal antibody 5F11 and doxorubicin (DXR), in the treatment of lung cancer cells and its resistance to a drug-resistant lung cancer cell line. Methods 5F11 DXR was prepared by glutaraldehyde method. Colony formation assay and dye exclusion test were used to evaluate the killing effect on sensitive target cells A2, resistant target cells 80 1 D and 80 1 DDXR, and non-target cells Ehrlich ascites carcinoma cells. . Results Various detection methods showed that the killing effect of 5F11 DXR on A2, 80 1 D and 80 1 DDXR cells was significantly higher than that of DXR alone (P <0.05) when the DXR content was 0.4 μg/ml; Especially for sensitive cell line A2 cells, this difference can be demonstrated at a lower concentration (0.04 μg/ml). However, there was no significant difference in the killing effect of Ehrlich ascites carcinoma cells on non-target cells (P > 0.05). Conclusion The killing effect of 5F11 DXR on sensitive target cells was significantly enhanced compared with DXR, and it could effectively reverse the multidrug resistance of DXR-resistant target cells. There was no significant difference in the killing effect of the two on non-target cells.