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在弱酸性条件下,甲基蓝(MB)可以通过静电和疏水作用聚集到血红蛋白(Hb)上并形成离子缔合物,不仅使得吸收光谱发生变化,而且使得共振瑞利散射增强。据此发展了一种测定Hb的RRS便捷方法,其线性范围和检出限分别为0.2~20μg/mL和0.012μg/mL。与RRS法相比,分光光度法的线性范围和检出限分别为8~60μg/mL和0.688μg/mL(344 nm),5~60μg/mL和0.398μg/mL(408 nm)。此RRS法可用于尿样中Hb的痕量检测。实验考察了体系的吸收、散射光谱性质和最佳聚合条件,并在最佳条件下研究了分析参数及作用机理。并初步讨论了有机小分子对体系的影响,结果表明分子间氢键是主要作用力不容忽视。
Under mildly acidic conditions, methyl blue (MB) can accumulate on hemoglobin (Hb) through electrostatic and hydrophobic interactions and form ion-association compounds, not only changing the absorption spectrum but also enhancing the resonance Rayleigh scattering. Therefore, a convenient RRS method for the determination of Hb was developed. The linear range and the detection limit were 0.2 ~ 20μg / mL and 0.012μg / mL, respectively. The linear range and the limit of detection of spectrophotometry were 8-60 μg / mL and 0.688 μg / mL (344 nm), 5-60 μg / mL and 0.398 μg / mL (408 nm), respectively, compared with the RRS method. This RRS method can be used for trace detection of Hb in urine samples. The absorption and scattering properties of the system and the optimal polymerization conditions were investigated experimentally, and the analytical parameters and mechanism were studied under the optimum conditions. The effects of small organic molecules on the system were discussed preliminarily. The results show that the intermolecular hydrogen bond is the main force that can not be neglected.